服務熱線:0531-68818568

聯係我們/CONTACT US

MORE +
版權所有:COPYRIGHT (©) 2018 濟南AG8亚游官网生物技術有限公司 版權所有
地址:濟南市曆城區二環東路2277號金橋國際大廈南樓1305室(洪家樓山大老校東臨)
電話:0531-68818568     18660191878
QQ:552392000
當前位置:主頁 > 產品中心 >
熒光封片劑(含核酸染料DAPI)
發布時間:2018-06-30 11:15   發布人:管理員
 Fluoroshield Mounting Medium With DAPI (20ml) (ab104139)
http://www.abcam.cn/fluoroshield-mounting-medium-with-dapi-20ml-ab104139.html
 
Tested applications     ICC/IF
General notes     Fluoroshield with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This unique formula prevents rapid photobleaching of FITC, Texas Red, AMCA, Cy2, Cy3, Cy5, Alexa fluoro 488, Alexa fluoro 594, Green fluorescent protein (GFP), tetramethyly rhodamine, Redox. Phycoerythrin (RP-E), Phyocyanin (PC), and Allophycocyanin (APC). Fluorescence is retained during prolonged storage at 4oC in the dark. This medium does not contain phenylenediamine, which destroys immunofluorescence of Cy dyes, RP-E, PC and APC. This mounting medium is fortified with DAPI which is a counter-stain for DNA. This product is to be used in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360nm and emits at 460nm, producing a blue fluorescence. RNA is also stained with DAPI. 
Procedure 
· Bring the vial to room temperature.
· Rinse slide to be mounted with distilled or deionized, touch the edges of slide on a paper towel to remove excess water. Place slides on a flat surface.
· Turn the vial upside down and open the dropper to remove any air bubbles.
· Apply 3-4 drops of mounting medium directly on top of the specimen and spread out evenly by tilting slide back and forth or spread evenly with a 0.2 ml plastic pipette tip making sure the tissue is not touched. Excess medium can be removed by touching the edges of slide against paper towel.
· Let stand at room temperature for about 5 minutes.
· Apply cover slip carefully avoiding air bubbles.
· The specimen is ready for visualization under a microscope.
· One can seal the edges of cover slip with nail polish, any organic medium or our Organo mounting medium (ab104141). If a coverslip is not sealed air bubbles will appear in few days.
· For storage it is recommended that the slide be stored in the dark at 2-8°C.
 
Removal of Coverslip 
Coverslip can be removed before sealing the edges. Soak slide in warm (37°C) distilled or deiononized water for several minutes. Carefully and slowly move the coverslip. Soak in water for an additional few minutes to remove coverslip. Rinse slide several times with warm water to remove all mounting medium. The slide can be remounted again.
 
Storage instructions    Store at +4°C in the dark. Do not freeze
Storage buffer     Preservative: 0.05% Sodium azide
Constituents: 84% Water, 0.0002% DAPI, 0.5% Proprietary component

版權所有:COPYRIGHT (©) 2018 濟南AG8亚游官网生物技術有限公司 版權所有 地址:濟南市曆城區二環東路2277號金橋國際大廈南樓1305室(洪家樓山大老校東臨)  電話:0531-68818568
技術支持:富庫網絡